Flow cytometry and cell sorting: A technique for analysis and sorting of aquatic particles1
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چکیده
منابع مشابه
Application of flow cytometry and cell sorting to megakaryocytopoiesis.
We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell frac...
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Dendritic cells (DC) are very specialized antigen presenting cells which play critical roles in innate and adaptive immunity and in tolerance. Their major functions are to sample antigens throughout the body and to stimulate or tolerize T cells. DC consist of several distinct subsets with different tissue distribution, surface markers, and function. This chapter discusses the lung DC subsets, t...
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Development of an improved technique for separating microbial cells from marine sediments and standardization of a high-throughput and discriminative cell enumeration method were conducted. We separated microbial cells from various types of marine sediment and then recovered the cells using multilayer density gradients of sodium polytungstate and/or Nycodenz, resulting in a notably higher perce...
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Cell size is a defining characteristic central to cell function and ultimately to tissue architecture. The ability to sort cell subpopulations of different sizes would facilitate investigation at genomic and proteomic levels of mechanisms by which cells attain and maintain their size. Currently available cell sorters, however, cannot directly measure cell volume electronically, and it would the...
متن کاملApplication of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples.
Flow cytometry (FCM) combined with viability staining is a useful tool in discerning viable bacteria in environmental samples where traditional culture methods may fail. Contamination of aerosol samples with dust and other non-biological particles can interfere with accurate sample analysis and therefore there is a desire to exclude those particles from analysis. Particles were sorted according...
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ژورنال
عنوان ژورنال: Limnology and Oceanography
سال: 1983
ISSN: 0024-3590
DOI: 10.4319/lo.1983.28.6.1275